Modulation of the antigenic phenotype of human breast carcinoma cells by...
SummaryIn the present study we have analyzed the effect of a synthetic protein kinase C (PKC) activator 3-(N-acetylamino)-5-(N-decyl-N-methylamino)-benzyl alcohol (ADMB) and the natural PKC-activating...
View ArticleDetachment of transformed cells
AbstractA parallel-plate flow chamber was used to quantify the detachment of normal cloned rat embryo fibroblasts (CREF) fibroblasts,ras-transformed CREF fibroblasts (CREF T24), and CREF T24...
View ArticleThe Role of Signal Transduction Pathways in Drug and Radiation Resistance
Abstract“MAPK” was first reported by Sturgill and Ray in 19861;. This protein kinase was originally described as a 42-kDa insulin-stimulated protein kinase activity whose tyrosine phosphorylation...
View ArticleThe Regulation of Tumor Suppressor Genes by Oncogenes
AbstractTumor suppressor genes (TSGs) and oncogenes represent the ying and the yang of cell growth, differentiation, and survival control. TSGs such as p53, the retinoblastoma (RB) gene product, and...
View ArticleIdentification of gene products suppressed by human immunodeficiency virus...
AbstractNeurodegeneration and human immunodeficiency virus type 1 (HIV-1)-associated dementia (HAD) are the major disease manifestations of HIV-1 colonization of the central nervous system (CNS). In...
View ArticleEffects of human immunodeficiency virus type 1 on astrocyte gene expression...
AbstractNeurodegeneration and dementia caused by human immunodeficiency virus type 1 (HIV-1) infection of the brain are common complications of acquired immunodeficiency syndrome (AIDS). Introduction...
View ArticleApproaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells
AbstractThis chapter will describe methods to assess the activities of protein kinases. Initial studies in the 1950s and 1960s in the field of glucose metabolism examined the activities of several...
View ArticleReciprocal Subtraction Differential RNA Display (RSDD)
AbstractIdentification of differentially expressed genes is an essential step in comprehending the molecular basis of complex physiological and pathological processes. Subtraction hybridization and...
View ArticleSurface-Epitope Masking (SEM)
AbstractAn immunological subtraction approach, surface-epitope masking (SEM), is described that permits the efficient and selective production of monoclonal antibodies (MAbs) reacting with both known...
View ArticleComplete Open Reading Frame (C-ORF) Technique
AbstractSeveral approaches, generally referred to as rapid amplification of cDNA ends, are currently used as a means of obtaining full-length cDNA clones by PCR. However, these protocols are not...
View ArticleCloning Differentially Expressed Genes Using Rapid Subtraction Hybridization...
AbstractDifferential gene expression represents the entry point for comprehending complex biological processes. In this context, identification and cloning of differentially expressed genes represent...
View ArticleCurrent advances in ER stress intervention therapies
AbstractThe endoplasmic reticulum (ER) is the site of synthesis and folding of membrane-localised and secretory proteins. The load upon ER client proteins that cells process varies considerably...
View ArticleSH3GL2 is frequently deleted in non-small cell lung cancer and downregulates...
AbstractThe purpose of this study was to identify key genetic pathways involved in non-small cell lung cancer (NSCLC) and understand their role in tumor progression. We performed a genome wide scanning...
View ArticleExpression patterns of MDA-9/syntenin during development of the mouse embryo
AbstractMelanoma differentiation associated gene-9 (MDA-9)/syntenin is a PDZ domain-containing adaptor protein involved in multiple diverse cellular processes including organization of protein...
View ArticleThe role of tumor-associated macrophages in tumor vascularization
AbstractTumor vascularization is a highly complex process that involves the interaction between tumors and their surrounding stroma, as well as many distinct angiogenesis-regulating factors. Tumor...
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